ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
32.20 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
HCRTR1, Hypocretin receptor type 1, Orexin receptor type 1, Orexin/Hypocretin receptor type 1, Ox-1-R, Ox1-R, Ox1R
背景
Orexin/Hypocretin receptor type 1 is a ~47 kDa protein. G-protein coupled receptor that binds the neuropeptide orexin-A with high affinity, and orexin-B with lower affinity, two peptides derived from a common precursor, prepro-orexin. Its activity is mediated via a G(q)-protein-coupled pathway, which activates the phosphatidylinositol-calcium second messenger system in response to orexin-A binding. In addition to G(q)-mediated signaling, orexin-A stimulation also promotes beta-arrestin recruitment, leading to receptor internalization. Plays a significant role in the regulation of food intake.
1. Hellmann, J. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 18059-18067. PMID: 32669442
2. Sakurai, T. et al. (1998) Cell 92, 573-85. PMID: 9491897
3. Milasta, S. et al. (2005) The Biochemical journal 387, 573-84. PMID: 15683363
