Human Leptin R Recombinant Protein (C-Fc) (HW637021)
概述
图片
参考文献
产品说明书
概述
货号
HW637021
品牌
abinScience
描述
Recombinant Human CD295/LEPR Protein, C-Fc (HW637021) expressed in Mammalian Cells, spanning Met1-Asp839. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P48357
蛋白长度
Met1-Asp839
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
122.44 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Leptin receptor is a ~132 kDa protein. Receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity. Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis.
1. Carpenter, B. et al. (2012) Structure (London, England : 1993) 20, 487-97. PMID: 22405007
2. Clément, K. et al. (1998) Nature 392, 398-401. PMID: 9537324
3. Zhao, Y. et al. (2003) Biochemical and biophysical research communications 300, 247-52. PMID: 12504075
4. Cassano, S. et al. (2014) Metabolism: clinical and experimental 63, 1272-9. PMID: 25060689
5. Bennett, BD. et al. (1996) Current biology : CB 6, 1170-80. PMID: 8805376
