ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
45.22 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
EC:2.7.9.3, SELD, SEPHS1, SPS, SPS1, Selenide, water dikinase 1, Selenium donor protein 1, Selenophosphate synthase 1
背景
Zincore component SEPHS1 is a ~42 kDa protein. Core component of the zincore complex, a heterotetramer that acts as a molecular 'grip' to stabilize transcription factors at DNA-binding sites across the genome, thereby controlling gene expression. The zincore complex binds specifically to zinc finger transcription factors, such as ZFP91, ZNF652, ZNF526 and PRDM15, and stabilizes them onto their cognate DNA motif. Within the complex, SEPHS1, recognizes and binds the backbone of zinc fingers of transcription factors in a sequence-independent manner via its arginine clamp, enhancing their DNA-binding stability. Plays an essential role in redox homeostasis. May also be involved in selenocysteine biosynthesis by catalyzing formation of selenophosphate from selenide and ATP.
1. Bianchi, D. et al. (2025) Science (New York, N.Y.) 389, eadv2861. PMID: 40608935
2. Lee, MO. et al. (2019) Biochemical and biophysical research communications 520, 406-412. PMID: 31607477
3. Low, SC. et al. (1995) The Journal of biological chemistry 270, 21659-64. PMID: 7665581
4. Tamura, T. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 16162-7. PMID: 15534230
