Mouse CYP11A1 Recombinant Protein (N-His) (MY252012)

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概述

货号	MY252012
品牌	abinScience
描述	Recombinant Mouse CYP11A1 Protein, N-His (MY252012) expressed in E. coli, spanning Thr37-Lys306. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	E. coli
Accession号	Q9QZ82
蛋白长度	Thr37-Lys306
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Mus musculus (Mouse)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	33.75 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	CYP11A, CYP11A1, CYPXIA1, Cholesterol desmolase, Cholesterol side-chain cleavage enzyme, mitochondrial, Cytochrome P450 11A1, Cytochrome P450(scc), EC:1.14.15.6
背景	Cholesterol side-chain cleavage enzyme, mitochondrial is a ~60 kDa protein. A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
Note	For research use only

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参考文献

产品说明书

Mouse CYP11A1 Recombinant Protein (N-His).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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质量

浓度

体积

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公式

C₁ × V₁ = C₂ × V₂

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母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

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Mouse CYP11A1 Recombinant Protein (N-His) (MY252012)

概述

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参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Mouse CYP11A1 Recombinant Protein (N-His) (MY252012)

概述

图片

参考文献

产品说明书

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