Mouse UNG Recombinant Protein (N-His) (MY404012)

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1mg

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概述

货号	MY404012
品牌	abinScience
描述	Recombinant Mouse UNG Protein, N-His (MY404012) expressed in E. coli, spanning Ser60-Leu306. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	E. coli
Accession号	P97931
蛋白长度	Ser60-Leu306
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Mus musculus (Mouse)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	30.14 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase
背景	Uracil-DNA glycosylase is a ~33 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication. 1. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144 2. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624 3. Rada, C. et al. (2002) Current biology : CB 12, 1748-55. PMID: 12401169 4. Maul, RW. et al. (2011) Nature immunology 12, 70-6. PMID: 21151102 5. Schrader, CE. et al. (2005) The Journal of experimental medicine 202, 561-8. PMID: 16103411
Note	For research use only

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参考文献

产品说明书

Mouse UNG Recombinant Protein (N-His).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

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Mouse UNG Recombinant Protein (N-His) (MY404012)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Mouse UNG Recombinant Protein (N-His) (MY404012)

概述

图片

参考文献

产品说明书

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