Human IL-6R alpha/IL6R Recombinant Protein (C-Fc) (HY583021)
概述
图片
参考文献
产品说明书
概述
货号
HY583021
品牌
abinScience
描述
Recombinant Human CD126/IL6R/IL-6RA Protein, C-Fc (HY583021) expressed in Mammalian Cells, spanning Met1-Pro365. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P08887
蛋白长度
Met1-Pro365
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
66.62 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interleukin-6 receptor subunit alpha is a ~51 kDa protein. Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system.
1. Larsen, JV. et al. (2017) Molecular and cellular biology 37. PMID: 28265003
2. Kang, S. et al. (2019) Immunity 50, 1007-1023. PMID: 30995492
3. Spencer, S. et al. (2019) The Journal of experimental medicine 216, 1986-1998. PMID: 31235509
Note
For research use only
图片
Bioactivity
Immobilized HY583021 Human CD126/IL6R/IL-6RA Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HY328011 Human IL6 Recombinant Protein (C-His-Avi), the EC 50 is 63.7 ng/mL.
Bioactivity
Immobilized HY583021 Human CD126/IL6R/IL-6RA Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HY328021 Human IL6 Recombinant Protein (C-His), the EC 50 is 115 ng/mL.
Bioactivity
Immobilized HY328011 Human IL6 Recombinant Protein (C-His-Avi) at 2 μg/mL(100 μL/well) can bind HY583021 Human CD126/IL6R/IL-6RA Recombinant Protein (C-Fc), the EC 50 is 222 ng/mL.
Bioactivity
Immobilized HY328021 Human IL6 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HY583021 Human CD126/IL6R/IL-6RA Recombinant Protein (C-Fc), the EC 50 is 162 ng/mL.
Bioactivity
Immobilized HY328041 Human IL6 Recombinant Protein (N-HSAD3 & C-His) at 2 μg/mL(100 μL/well) can bind HY583021 Human CD126/IL6R/IL-6RA Recombinant Protein (C-Fc), the EC 50 is 133 ng/mL.
Bioactivity
Immobilized HY328051 Human IL6 Recombinant Protein (C-Strep) at 2 μg/mL(100 μL/well) can bind HY583021 Human CD126/IL6R/IL-6RA Recombinant Protein (C-Fc), the EC 50 is 267 ng/mL.
