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Yersinia enterocolitica sctE/YopB Recombinant Protein (N-His) (JN174012)

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概述
货号JN174012
品牌abinScience
描述
Recombinant Yersinia enterocolitica sctE/YopB Protein, N-His (JN174012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
表达系统E. coli
Accession号P37131
蛋白长度Gly246-Val401
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Yersinia enterocolitica
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名T3SS translocon protein SctE, Translocator YopB, Type 3 secretion system translocon protein SctE, Yersinia outer protein B, sctE, yopB
背景

Type 3 secretion system translocon protein SctE is a ~41 kDa protein. Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. YopB/SctE and YopD/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells. Is an essential virulence determinant. Required for YopE translocation.

1. Neyt, C. et al. (1999) Molecular microbiology 33, 971-81. PMID: 10476031
2. Tardy, F. et al. (1999) The EMBO journal 18, 6793-9. PMID: 10581252
3. Lee, VT. et al. (1999) Molecular microbiology 31, 1619-29. PMID: 10209737
4. Nordfelth, R. et al. (2001) Infection and immunity 69, 3516-8. PMID: 11292787
NoteFor research use only
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参考文献
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公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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