ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 is a ~138 kDa protein. Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Required for correct mitotic spindle orientation and therefore progression of mitosis. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity.
1. Vandeput, F. et al. (2006) Cellular signalling 18, 2193-9. PMID: 16824732
2. Habib, T. et al. (1998) The Journal of biological chemistry 273, 18605-9. PMID: 9660833
3. Dyson, JM. et al. (2001) The Journal of cell biology 155, 1065-79. PMID: 11739414
4. Dyson, JM. et al. (2003) Blood 102, 940-8. PMID: 12676785
5. Prasad, NK. et al. (2005) The Journal of biological chemistry 280, 13129-36. PMID: 15668240
6. Hasegawa, J. et al. (2011) The Journal of cell biology 193, 901-16. PMID: 21624956
7. Prasad, N. et al. (2002) Journal of cell science 115, 3807-15. PMID: 12235291
8. Koch, A. et al. (2005) Oncogene 24, 3436-47. PMID: 15735664
9. Zhuang, G. et al. (2007) The Journal of biological chemistry 282, 2683-94. PMID: 17135240
10. Pengal, RA. et al. (2003) The Journal of biological chemistry 278, 22657-63. PMID: 12690104
