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Mouse LRRK2/Dardarin Recombinant Protein (N-His) (MV789012)

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  • 50ug
  • 100ug
  • 1mg
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概述
货号MV789012
品牌abinScience
描述
Recombinant Mouse LRRK2/Dardarin Protein, N-His (MV789012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
表达系统E. coli
Accession号Q5S006
蛋白长度Ile2151-Leu2500
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Mus musculus (Mouse)
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名EC:2.7.11.1, EC:3.6.5.-, Leucine-rich repeat serine/threonine-protein kinase 2, Lrrk2
背景

Leucine-rich repeat serine/threonine-protein kinase 2 is a ~284 kDa protein. Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation. Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43. Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A. Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A.

1. Steger, M. et al. (2016) eLife 5. PMID: 26824392
2. Chen, ZC. et al. (2017) Science signaling 10. PMID: 28720718
3. Steger, M. et al. (2017) eLife 6. PMID: 29125462
4. Purlyte, E. et al. (2018) The EMBO journal 37, 1-18. PMID: 29212815
5. Dhekne, HS. et al. (2018) eLife 7. PMID: 30398148
6. Cho, HJ. et al. (2014) The EMBO journal 33, 2314-31. PMID: 25201882
7. Eguchi, T. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E9115-E9124. PMID: 30209220
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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