ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Midkine is a ~15 kDa protein. Secreted protein that functions as a cytokine and growth factor and mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptors via their chondroitin sulfate (CS) groups. Thereby regulates many processes like inflammatory response, cell proliferation, cell adhesion, cell growth, cell survival, tissue regeneration, cell differentiation and cell migration. Participates in inflammatory processes by exerting two different activities. Firstly, mediates neutrophils and macrophages recruitment to the sites of inflammation both by direct action by cooperating namely with ITGB2 via LRP1 and by inducing chemokine expression.
1. Ichihara-Tanaka, K. et al. (2006) The Journal of biological chemistry 281, 30857-64. PMID: 16901907
2. Zou, P. et al. (2006) Journal of neurochemistry 99, 1470-9. PMID: 17230638
3. Horiba, M. et al. (2000) The Journal of clinical investigation 105, 489-95. PMID: 10683378
4. Ochiai, K. et al. (2004) Liver international : official journal of the International Association for the Study of the Liver 24, 484-91. PMID: 15482347
5. Kawai, H. et al. (2004) The American journal of pathology 165, 1603-12. PMID: 15509530
6. Horiba, M. et al. (2006) Circulation 114, 1713-20. PMID: 17015789
7. Takenaka, H. et al. (2009) American journal of physiology. Heart and circulatory physiology 296, H462-9. PMID: 19060126
