ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
38.39 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
IBP-5, IBP5, IGF-binding protein 5, IGFBP-5, IGFBP5, Insulin-like growth factor-binding protein 5
背景
Insulin-like growth factor-binding protein 5 is a ~30 kDa protein. Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells. Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling. Also plays a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs.
1. Busby, WH. et al. (2009) Osteoarthritis and cartilage 17, 547-55. PMID: 18930415
2. Jones, JI. et al. (1993) The Journal of cell biology 121, 679-87. PMID: 7683690
3. Yamaguchi, Y. et al. (2011) Journal of cellular and molecular medicine 15, 957-69. PMID: 20345844
4. Su, Y. et al. (2015) PloS one 10, e0130546. PMID: 26103640
5. Lin, W. et al. (2023) Nature communications 14, 1578. PMID: 36949068
