ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
56.02 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Thromboxane-A synthase is a ~60 kDa protein. Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation. Also cleaves PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2. Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE.
1. Hsu, PY. et al. (2000) Archives of biochemistry and biophysics 383, 119-27. PMID: 11097184
2. Wang, LH. et al. (2001) The Journal of biological chemistry 276, 14737-43. PMID: 11297515
3. Chen, CY. et al. (2012) Pharmacogenetics and genomics 22, 653-8. PMID: 22735388
4. Matsunobu, T. et al. (2013) Journal of lipid research 54, 2979-87. PMID: 24009185
5. Yokoyama, C. et al. (1993) FEBS letters 318, 91-4. PMID: 8436233
6. Hsu, PY. et al. (1999) The Journal of biological chemistry 274, 762-9. PMID: 9873013
7. Yeh, HC. et al. (2007) Archives of biochemistry and biophysics 461, 159-68. PMID: 17459323
