ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
14.65 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
G11, Inactive serine/threonine-protein kinase 19, Protein G11, Protein RP1, RP1, STK19, TWH19, Tandem winged helix protein formerly known as STK19, WHR1, Winged helix repair factor 1
背景
Winged helix repair factor 1 is a ~28 kDa protein. DNA-binding protein which is required for efficient transcription-coupled nucleotide excision repair (TC-NER). Acts as part of a TC-NER complex which assembles and interacts with RNA polymerase II (RNAPII) when it stalls at DNA lesions. TC-NER complex subunit UVSSA binds to the GTF2H1/p62 subunit of the TFIIH transcription factor complex, tethering TFIIH to the TC-NER complex. WHR1/STK19 then interacts with the XPD helicase subunit of TFIIH which guides TFIIH to DNA downstream of the stalled RNAPII, ensuring DNA repair. Directly interacts with RNAPII and also binds to downstream DNA.
1. Li, Y. et al. (2024) The Journal of cell biology 223. PMID: 38252411
2. Li, J. et al. (2024) Scientific reports 14, 14098. PMID: 38890355
3. Tan, Y. et al. (2024) Nucleic acids research 52, 12767-12783. PMID: 39353615
