ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
EC:2.1.1.357, Histone-lysine N-methyltransferase NSD2, KIAA1090, MMSET, Multiple myeloma SET domain-containing protein, NSD2, Nuclear SET domain-containing protein 2, Protein trithorax-5, TRX5, WHSC1, Wolf-Hirschhorn syndrome candidate 1 protein
背景
Histone-lysine N-methyltransferase NSD2 is a ~152 kDa protein. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro. Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells. By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes.
1. Li, Y. et al. (2009) The Journal of biological chemistry 284, 34283-95. PMID: 19808676
2. Kuo, AJ. et al. (2011) Molecular cell 44, 609-20. PMID: 22099308
3. Tisi, D. et al. (2016) ACS chemical biology 11, 3093-3105. PMID: 27571355
4. Zhuang, L. et al. (2018) Nature communications 9, 1796. PMID: 29728617
5. Zanoni, P. et al. (2021) Genetics in medicine : official journal of the American College of Medical Genetics 23, 1474-1483. PMID: 33941880
6. Hudlebusch, HR. et al. (2005) British journal of haematology 130, 700-8. PMID: 16115125
