ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
17.29 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH-I, MARCH1, MARCHF1, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, RING finger protein 171, RING-type E3 ubiquitin transferase MARCHF1, RNF171
背景
E3 ubiquitin-protein ligase MARCHF1 is a ~32 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system. Also regulates insulin sensitivity by controlling surface expression of the insulin receptor subunit beta/INSR by direct ubiquitination and degradation.
1. Thibodeau, J. et al. (2008) European journal of immunology 38, 1225-30. PMID: 18389477
2. De Gassart, A. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 3491-6. PMID: 18305173
3. Tze, LE. et al. (2011) The Journal of experimental medicine 208, 149-65. PMID: 21220452
4. Martin, M. et al. (2022) Journal of virology 96, e0180621. PMID: 35045264
5. Nagarajan, A. et al. (2016) Nature communications 7, 12639. PMID: 27577745
