ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
56.55 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Ubiquitin carboxyl-terminal hydrolase 39 is a ~65 kDa protein. Deubiquitinating enzyme that plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development. Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome. Specifically regulates immunoglobulin gene rearrangement in a spliceosome-dependent manner, which involves modulating chromatin interactions at the Igh locus and therefore plays an essential role in B-cell development. Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint. Regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2.
1. Peng, Y. et al. (2020) Journal of immunology (Baltimore, Md. : 1950) 205, 3167-3178. PMID: 33127822
2. Kim, JJ. et al. (2021) Nucleic acids research 49, 11083-11102. PMID: 34614178
3. Makarova, OV. et al. (2001) The EMBO journal 20, 2553-63. PMID: 11350945
4. Agafonov, DE. et al. (2016) Science (New York, N.Y.) 351, 1416-20. PMID: 26912367
5. van Leuken, RJ. et al. (2008) Cell cycle (Georgetown, Tex.) 7, 2710-9. PMID: 18728397
6. Wu, J. et al. (2019) Cancer letters 449, 114-124. PMID: 30771428
7. Quan, J. et al. (2023) Journal of immunology (Baltimore, Md. : 1950) 210, 640-652. PMID: 36651806
