ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
14.76 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Podoplanin is a ~18 kDa protein. Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation. Interaction with CD9, on the contrary, attenuates platelet aggregation and pulmonary metastasis induced by PDPN. Mediates effects on cell migration and adhesion through its different partners. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness.
1. Kato, Y. et al. (2003) The Journal of biological chemistry 278, 51599-605. PMID: 14522983
2. Kaneko, M. et al. (2004) The Journal of biological chemistry 279, 38838-43. PMID: 15231832
3. Suzuki-Inoue, K. et al. (2007) The Journal of biological chemistry 282, 25993-6001. PMID: 17616532
4. Uhrin, P. et al. (2010) Blood 115, 3997-4005. PMID: 20110424
8. Boucherot, A. et al. (2002) Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 17, 978-84. PMID: 12032185
