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GMP Grade Bovine FGF basic/FGF2/bFGF (Tag Free) (BY398012)

价格: --
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
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  • 参考文献

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概述
货号BY398012
品牌abinScience
描述
GMP Grade Bovine FGF basic/FGF2/bFGF (Tag Free) (BY398012) expressed in E. coli. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
  • ●Recombinant — Consistent lot-to-lot performance.
  • ●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
  • ●Bioactive — Verified biological activity.
表达系统E. coli
Accession号P03969
蛋白长度Pro10-Ser155
应用ELISA, Functional assay
种属Bos taurus (Bovine)
生物活性Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL.
性质Recombinant
内毒素水平<0.01 EU/ug
纯度>95% as determined by SDS-PAGE.
预测分子量17.4 kDa
状态Lyophilized
保存溶液 Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 250 mM NaCl, 5% Mannitol, 0.01% Tween 80, pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Basic fibroblast growth factor, FGF-2, FGF2, Fibroblast growth factor 2, HBGF-2, Heparin-binding growth factor 2, Kidney-derived growth factor, bFGF
背景

Fibroblast growth factor 2 (FGF2/bFGF) is a ~30 kDa protein. Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro.

1. Ornitz, DM. et al. (1996) The Journal of biological chemistry 271, 15292-7. PMID: 8663044
2. Mori, S. et al. (2017) Bioscience reports 37. PMID: 28302677
3. Shimoyama, Y. et al. (1991) Japanese journal of cancer research : Gann 82, 1263-70. PMID: 1721615
4. Gautschi, P. et al. (1986) FEBS letters 204, 203-7. PMID: 3732516
5. Gimenez-Gallego, G. et al. (1986) Biochemical and biophysical research communications 135, 541-8. PMID: 3964259
6. Mori, S. et al. (2013) PloS one 8, e57927. PMID: 23469107
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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