GMP Grade Human Neuregulin-1-beta 1/NRG1-beta 1 (Tag Free) (HX096022)
概述
图片
参考文献
产品说明书
概述
货号
HX096022
品牌
abinScience
描述
Human Neuregulin-1-beta 1/NRG1-beta 1 Recombinant Protein (Tag Free) (HX096022) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
●Recombinant — Consistent lot-to-lot performance.
●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
●Bioactive — Verified biological activity.
表达系统
Mammalian cells
Accession号
Q02297-6
蛋白长度
Ser177-Glu241
应用
ELISA, Functional assay
种属
Homo sapiens (Human)
生物活性
Measured in a cell proliferation assay using MCF-7 breast cancer cell line.The ED50 for this effect is 1-8 ng/mL.
性质
Recombinant
内毒素水平
<0.01 EU/ug
纯度
>95% as determined by SDS-PAGE.
预测分子量
7.5 kDa
状态
Lyophilized
保存溶液
Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose, 0.01% Tween 80, pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
ARIA, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, GGF, Glial growth factor, HGL, HRG, HRGA, Heregulin, NDF, NRG1, Neu differentiation factor, Neuregulin-1, Pro-NRG1, Pro-neuregulin-1, membrane-bound isoform, SMDF, Sensory and motor neuron-derived factor
背景
Pro-neuregulin-1, membrane-bound isoform (NRG1) is a ~70 kDa protein. Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4.
1. Sweeney, C. et al. (2000) The Journal of biological chemistry 275, 19803-7. PMID: 10867024
