GMP Grade Human IL-22 (Tag Free) (HV999011) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
●Recombinant — Consistent lot-to-lot performance.
●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
●Bioactive — Verified biological activity.
表达系统
Mammalian cells
Accession号
Q9GZX6
蛋白长度
Ala34-Ile179
应用
ELISA, Functional assay
种属
Homo sapiens (Human)
生物活性
Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is ≤2.
性质
Recombinant
内毒素水平
<0.01 EU/ug
纯度
>95% as determined by SDS-PAGE.
预测分子量
16.5 kDa
状态
Lyophilized
保存溶液
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interleukin-22 (Interleukin-22,Cytokine Zcyto18,IL-22,ZCYTO18,IL22,ILTIF,IL-TIF,IL-10-related T-cell-derived-inducible factor) is a ~20 kDa protein. Cytokine that plays a critical role in modulating tissue responses during inflammation. Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage. Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3.
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4. Akil, H. et al. (2015) PloS one 10, e0119872. PMID: 25793261
5. Moniruzzaman, M. et al. (2019) International journal of molecular sciences 20. PMID: 31311100
