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GMP Grade Human EGF (Tag Free) (HF557021)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HF557021
品牌abinScience
描述
Human EGF Recombinant Protein (Tag Free) (HF557021) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
  • ●Recombinant — Consistent lot-to-lot performance.
  • ●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
  • ●Bioactive — Verified biological activity.
表达系统Mammalian cells
Accession号P01133
蛋白长度Asn971-Arg1023
应用ELISA, Functional assay
种属Homo sapiens (Human)
生物活性Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤0.3 ng/mL.
性质Recombinant
内毒素水平<0.01 EU/ug
纯度>95% as determined by SDS-PAGE.
预测分子量6.2 kDa
状态Lyophilized
保存溶液 Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名EGF, Epidermal growth factor, Pro-epidermal growth factor, Urogastrone
背景

Pro-epidermal growth factor (EGF) is a ~133 kDa protein. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.

1. Hermann, PM. et al. (2000) The Journal of neuroscience : the official journal of the Society for Neuroscience 20, 6355-64. PMID: 10964941
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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