GMP Grade Human HGF (Tag Free) (HB191021) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
●Recombinant — Consistent lot-to-lot performance.
●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
●Bioactive — Verified biological activity.
表达系统
Mammalian cells
Accession号
P14210
蛋白长度
α chain：Gln32-Arg494 & β chain：Val495-Ser728
应用
ELISA, Functional assay
种属
Homo sapiens (Human)
生物活性
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is ≤2.5 ng/ml..
性质
Recombinant
内毒素水平
<0.01 EU/ug
纯度
>95% as determined by SDS-PAGE.
预测分子量
53.7 kDa ( α chain), 26 kDa ( β chain)
状态
Lyophilized
保存溶液
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Hepatocyte growth factor (SF,HPTA,Hepatopoietin-A,Hepatocyte growth factor,Scatter factor,HGF) is a ~83 kDa protein. Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.
1. Tolbert, WD. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13264-9. PMID: 20624990
2. Stamos, J. et al. (2004) The EMBO journal 23, 2325-35. PMID: 15167892
3. Hashimoto, T. et al. (2010) The FEBS journal 277, 4888-900. PMID: 20977675
