GMP Grade Human IL-33 (Tag Free) (HF543032) expressed in E. coli. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
●Recombinant — Consistent lot-to-lot performance.
●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
●Bioactive — Verified biological activity.
表达系统
E. coli
Accession号
O95760
蛋白长度
Ser112-Thr270
应用
ELISA, Functional assay
种属
Homo sapiens (Human)
生物活性
Measured by its ability to induce IL-8 secretion by HUVEC human umbilic vein endothelial cells. The ED50 for this effect is ≤1.0ng/ml.
性质
Recombinant
内毒素水平
<0.01 EU/ug
纯度
>95% as determined by SDS-PAGE.
预测分子量
18.1 kDa
状态
Lyophilized
保存溶液
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
C9orf26, IL-1F11, IL-33, IL1F11, IL33, Interleukin-1 family member 11, Interleukin-33, Interleukin-33 (109-270), Interleukin-33 (95-270), Interleukin-33 (99-270), NF-HEV, NFHEV, Nuclear factor from high endothelial venules
背景
Interleukin-33 (NF-HEV,IL-1F11,C9orf26,IL-33,Interleukin-1 family member 11,IL33,Nuclear factor from high endothelial venules,NFHEV,Interleukin-33,IL1F11) is a ~30 kDa protein. Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as an enhancer of polarization of alternatively activated macrophages. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.
1. Schmitz, J. et al. (2005) Immunity 23, 479-90. PMID: 16286016
2. Kurowska-Stolarska, M. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 183, 6469-77. PMID: 19841166
3. Komai-Koma, M. et al. (2007) European journal of immunology 37, 2779-86. PMID: 17853410
4. Moussion, C. et al. (2008) PloS one 3, e3331. PMID: 18836528
