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GMP Grade Human PDGF-B (Tag Free) (HF877011)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HF877011
品牌abinScience
描述
Human PDGF-B Recombinant Protein (Tag Free) (HF877011) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
  • ●Recombinant — Consistent lot-to-lot performance.
  • ●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
  • ●Bioactive — Verified biological activity.
表达系统Mammalian cells
Accession号P01127
蛋白长度Ser82-Thr190
应用ELISA, Functional assay
种属Homo sapiens (Human)
生物活性Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤5 ng/mL.
性质Recombinant
内毒素水平<0.01 EU/ug
纯度>95% as determined by SDS-PAGE.
预测分子量12.2 kDa
状态Lyophilized
保存溶液 Lyophilized from a 0.22 μm-filtered solution containing PBS,5% Mannitol, 0.01% Tween 80, pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Becaplermin, PDGF subunit B, PDGF-2, PDGF2, PDGFB, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Platelet-derived growth factor subunit B, Proto-oncogene c-Sis, SIS
背景

Platelet-derived growth factor subunit B (PDGFB) is a ~27 kDa protein. Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing.

1. Vanlandewijck, M. et al. (2015) PloS one 10, e0143407. PMID: 26599395
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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