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Human SLC1A7 Recombinant Protein (N-His) (HT267012)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HT267012
品牌abinScience
描述
Recombinant Human SLC1A7 Protein, N-His (HT267012) expressed in E. coli, spanning His115-Thr216. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
表达系统E. coli
Accession号O00341
蛋白长度His115-Thr216
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Homo sapiens (Human)
性质Recombinant
内毒素水平Remove endotoxin and sterilization is required for cell assay.
纯度>90% as determined by SDS-PAGE.
预测分子量13.45 kDa
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名EAAT5, SLC1A7, Excitatory amino acid transporter 5, Solute carrier family 1 member 7, Retinal glutamate transporter
背景

Excitatory amino acid transporter 5 (SLC1A7) is a ~60 kDa protein. Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Acts primarily as an inhibitory glutamate-gated chloride channel being a major inhibitory presynaptic receptor at mammalian rod bipolar cell axon terminals. Glutamate binding gates a large Cl(-) conductance that mediates inhibition, affecting visual processing in the retina.

1. Arriza, JL. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 4155-60. PMID: 9108121
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Recombinant Human SLC1A7 Protein, N-His

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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