InVivoMAb Anti-Mouse IL-2 Antibody (JES6-1A12) (MX011010)

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规格：

100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

概述

货号

MX011010

品牌

abinScience

描述

InvivoMab Anti-Mouse IL2 Antibody (JES6-1A12) [JES6-1A12] (MX011010) is a rat monoclonal antibody detecting Aldesleukin in ELISA, FCM. Suitable for Mouse.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

种属反应性

Mouse

应用

ELISA, FCM

同种型

IgG2b, kappa

克隆号

JES6-1A12

靶标

Aldesleukin, IL-2, IL2, Interleukin-2, T-cell growth factor, TCGF

内毒素水平

< 1 EU/mg

纯度

>95% as determined by SDS-PAGE.

纯化方式

Protein A/G purified from cell culture supernatant.

Accession号

P60568

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Interleukin-2 (IL2) is a ~17 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways.

1. Mingari, MC. et al. (1984) Nature 312, 641-3. PMID: 6438535

2. Wang, X. et al. (2005) Science (New York, N.Y.) 310, 1159-63. PMID: 16293754

3. Stauber, DJ. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 2788-93. PMID: 16477002

4. Miyazaki, T. et al. (1994) Science (New York, N.Y.) 266, 1045-7. PMID: 7973659

5. Kirken, RA. et al. (1995) Cytokine 7, 689-700. PMID: 8580378

6. Osinalde, N. et al. (2015) Proteomics 15, 520-31. PMID: 25142963

7. Holbrook, NJ. et al. (1984) Proceedings of the National Academy of Sciences of the United States of America 81, 1634-8. PMID: 6608729

Note

For research use only.

Isotype Control

Rat IgG2b Recombinant Isotype Control Antibody (LTF-2) (RF974107)

图片

SDS-PAGE

SDS-PAGE for InvivoMab Anti-Mouse IL2 Antibody (JES6-1A12)

参考文献

产品说明书

InVivoMAb Anti-Mouse IL-2 Antibody (JES6-1A12).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

InVivoMAb Anti-Mouse IL-2 Antibody (JES6-1A12) (MX011010)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

InVivoMAb Anti-Mouse IL-2 Antibody (JES6-1A12) (MX011010)

概述

图片

参考文献

产品说明书

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