Anti-Human PCSK9 Recombinant Nanobody (SAA0995) (HV275023)

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50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HV275023

品牌

abinScience

描述

Anti-Human PCSK9 Nanobody (SAA0995) [SAA0995] (HV275023) is a alpaca monoclonal antibody detecting PCSK9 in ELISA. Suitable for Human.

种属反应性

Human

应用

ELISA

宿主

Alpaca

克隆类型

Monoclonal

同种型

VHH-8His-Cys-tag

克隆号

SAA0995

靶标

EC:3.4.21.-, NARC-1, NARC1, Neural apoptosis-regulated convertase 1, PC9, PCSK9, Proprotein convertase 9, Proprotein convertase subtilisin/kexin type 9, Subtilisin/kexin-like protease PC9

内毒素水平

< 10 EU/mg as determined by LAL method.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

Q8NBP7

状态

Liquid

保存溶液

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a ~74 kDa protein. Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. PCSK9 is the therapeutic target of evolocumab (Repatha).

1. Poirier, S. et al. (2008) The Journal of biological chemistry 283, 2363-72. PMID: 18039658

2. Nassoury, N. et al. (2007) Traffic (Copenhagen, Denmark) 8, 718-32. PMID: 17461796

3. Fan, D. et al. (2008) Biochemistry 47, 1631-9. PMID: 18197702

4. Mayer, G. et al. (2008) The Journal of biological chemistry 283, 31791-801. PMID: 18799458

5. Chen, Y. et al. (2011) Biochemical and biophysical research communications 415, 515-8. PMID: 22074827

6. Jonas, MC. et al. (2008) EMBO reports 9, 916-22. PMID: 18660751

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human PCSK9 Nanobody (SAA0995)

参考文献

产品说明书

Anti-Human PCSK9 Recombinant Nanobody (SAA0995).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PCSK9 Recombinant Nanobody (SAA0995) (HV275023)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PCSK9 Recombinant Nanobody (SAA0995) (HV275023)

概述

图片

参考文献

产品说明书

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