Anti-Human CASP3 Recombinant Nanobody (VHH2) (HW555033)

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50ug
100ug
1mg

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概述
图片
参考文献
产品说明书

概述

货号

HW555033

品牌

abinScience

描述

Anti-Human CASP3 Nanobody (VHH2) [VHH2] (HW555033) is a alpaca monoclonal antibody detecting CASP3 in ELISA. Suitable for Human.

种属反应性

Human

应用

ELISA

宿主

Alpaca

同种型

VHH-8His-Cys-tag

克隆号

VHH2

克隆类型

Monoclonal

靶标

Caspase-3, CPP-32, CPP32, CASP-3, SREBP cleavage activity 1, CASP3, Apopain, SCA-1, Cysteine protease CPP32, Protein Yama

内毒素水平

Please contact with the lab for this information.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Protein A/G purified from cell culture supernatant.

Accession号

P42574

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Caspase-3 (CASP3) is a ~31 kDa protein. Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively).

1. Walsh, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12815-9. PMID: 18723680

2. Nakatsumi, H. et al. (2010) The Journal of biological chemistry 285, 25418-25. PMID: 20566630

3. Thomsen, ND. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8477-82. PMID: 23650375

4. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844

5. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120

6. Nicholson, DW. et al. (1995) Nature 376, 37-43. PMID: 7596430

10. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human CASP3 Nanobody (VHH2)

参考文献

产品说明书

Anti-Human CASP3 Recombinant Nanobody (VHH2).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CASP3 Recombinant Nanobody (VHH2) (HW555033)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CASP3 Recombinant Nanobody (VHH2) (HW555033)

概述

图片

参考文献

产品说明书

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