Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242) (HY057033)

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100ug
1mg

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概述
图片
参考文献
产品说明书

概述

货号

HY057033

品牌

abinScience

描述

Anti-Human CD3E Nanobody (SAA2242) [SAA2242] (HY057033) is a human monoclonal antibody detecting CD3e in ELISA, FCM. Suitable for Human.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

种属反应性

Human

应用

ELISA, FCM

宿主

Human

同种型

VHH-hFc

克隆号

SAA2242

克隆类型

Monoclonal

经测试应用

FCM

靶标

T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain

内毒素水平

Please contact with the lab for this information.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P07766

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto).

1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938

2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002

3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325

5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human CD3E Nanobody (SAA2242)
Flow cytometry

Flow-cytometry using anti-human CD3E antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti- human CD3E monoclonal antibody (Catalog: HY057033,Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant human CD3E (Catalog No: HY057031) in indirect ELISA.

参考文献

产品说明书

Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242) (HY057033)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242) (HY057033)

概述

图片

参考文献

产品说明书

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