Bulk Anti-Myc Tag (9E10) Antibody (ICH1213-5mg)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

This bulk anti-Myc Tag antibody uses clone 9E10, one of the most widely adopted monoclonal reagents for detecting Myc-epitope-tagged recombinant proteins. It is a mouse IgG1 antibody produced in a CHO expression system and recognizes the c-Myc-derived peptide epitope (EQKLISEEDL) commonly appended to engineered proteins. Because the epitope is a short defined tag rather than a native cell-surface receptor, the reagent is suited to a broad range of detection and biochemistry workflows rather than to in-vivo functional modulation. The antibody is validated across an unusually wide application set, including ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification when conjugated to agarose or magnetic beads, and use as an isotype control. Offered as a low-endotoxin, bulk (mg to gram scale), research-use-only preparation, it supports laboratories needing consistent lot performance across large-scale tag-detection, affinity-capture, and screening pipelines where reagent uniformity and cost per assay matter. C-MYC (UniProt P01106) is a basic helix-loop-helix leucine-zipper transcription factor and a master regulator of cell growth, proliferation, metabolism, ribosome biogenesis, and apoptosis. It heterodimerizes with MAX to bind E-box DNA elements and drives transcription of thousands of genes, while also repressing targets through interactions with MIZ1 and other partners. MYC activity is normally tightly controlled by growth signals and is short-lived, being rapidly degraded via the ubiquitin-proteasome pathway. Dysregulated or amplified MYC is one of the most frequently deregulated drivers across human cancers, sustaining a proliferative and biosynthetic program. In the context of this reagent, the relevant feature is the well-characterized c-Myc-derived peptide epitope (residues around EQKLISEEDL), which is exploited as a genetically encoded "Myc tag" fused to recombinant proteins to enable specific detection, immunoprecipitation, and affinity purification independent of the protein's own antigenicity.