首页 > 产品中心 > 重组蛋白 >

PNGase F (JN842012)

价格:
规格:
  • 15KU
  • 75KU
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号JN842012
品牌abinScience
表达系统E. coli
蛋白长度PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
应用Removal of high mannose N-glycans from glycoproteins
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
状态Liquid
保存溶液 20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名CAS: 83534-39-8
文献1. Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31(1):2-7. [JN842012]
2. Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87(20):10199-204. [JN842012]
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for PNGase F

  • Bioactivity

    Deglycosylation of Human IgG1 Fc by PNGase F.

  • Bioactivity

    Schematic diagram of PNGase F cleavage site.

参考文献
产品说明书
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

相关产品推荐