| 货号 | JN842012 |
|---|---|
| 品牌 | abinScience |
| 描述 | Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins |
| 表达系统 | E. coli |
| 蛋白长度 | PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli. |
| 应用 | Removal of high mannose N-glycans from glycoproteins |
| 种属 | Elizabethkingia miricola |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 预测分子量 | 37.08 kDa |
| 状态 | Liquid |
| 保存溶液 | 20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| 别名 | 83534-39-8 |
| Note | For research use only. |
SDS-PAGE for PNGase F
Deglycosylation of Human IgG1 Fc by PNGase F.
Schematic diagram of PNGase F cleavage site.
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