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HEK293T Human GPRC5D Stable Cell Line (HP458818)

价格:
规格:
  • 1 ml
数量:
  • 概述

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  • 参考文献

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概述
货号HP458818
品牌abinScience
描述
HEK293T Human GPRC5D Stable Cell Line (HP458818) is a stable cell line expressing GPRC5D. Engineered for consistent, high-level target expression in research applications.
Highlights
  • Stable Expression — Engineered for consistent, high-level target expression.
Accession号Q9NZD1
应用FCM
种属Human
靶标GPRC5D
检测方法Colorimetric
样本类型Plasma, Serum
实验类型Quantitative
检测范围1.56 - 100 ng/mL
灵敏度0.25 ng/mL
回收率80-120%
性能Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
运输2-8 ℃
稳定性和存储Frozen in liquid nitrogen.
规格1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景

G-protein coupled receptor family C group 5 member D (GPRC5D) is a ~38 kDa protein. G-protein coupled receptor involved in hard keratin expression and likely plays a role in the development of hair and nails.

NoteFor research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.
图片
  • Flow cytometry

    Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPRC5D monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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