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HEK293T Human CD196/CCR6 Stable Cell Line (HW383818)

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规格：
  • 1 ml
数量：
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概述
货号HW383818
品牌abinScience
描述
HEK293T Human CD196/CCR6 Stable Cell Line (HW383818) is a stable cell line expressing CD196/CCR6. Engineered for consistent, high-level target expression in research applications.
Highlights
  • ●Stable Expression — Engineered for consistent, high-level target expression.
Accession号P51684
应用FCM
种属Human
靶标CD196/CCR6
性能Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
稳定性和存储Frozen in liquid nitrogen.
规格1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景

C-C chemokine receptor type 6 (CD196/CCR6) is a ~42 kDa protein. Receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins. Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity.

1. Baba, M. et al. (1997) The Journal of biological chemistry 272, 14893-8. PMID: 9169459
2. Röhrl, J. et al. (2010) The Journal of biological chemistry 285, 7028-34. PMID: 20068036
3. Lee, AY. et al. (2015) Cytokine 72, 97-101. PMID: 25585877
4. Diao, R. et al. (2014) Science translational medicine 6, 249ra108. PMID: 25122636
5. Ito, T. et al. (2011) Experimental cell research 317, 613-9. PMID: 21376174
6. Caballero-Campo, P. et al. (2014) Journal of cellular physiology 229, 68-78. PMID: 23765988
图片

  • Flow cytometry

    Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD196 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
产品说明书
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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HEK293T Human CD196/CCR6 Stable Cell Line

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