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HEK293T Human CD221/IGF1R Stable Cell Line (HY353818)

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  • 1 ml
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概述
货号HY353818
品牌abinScience
描述
HEK293T Human CD221/IGF1R Stable Cell Line (HY353818) is a stable cell line expressing CD221/IGF1R. Engineered for consistent, high-level target expression in research applications.
Highlights
  • ●Stable Expression — Engineered for consistent, high-level target expression.
Accession号P08069
应用FCM
种属Human
靶标CD221/IGF1R
性能Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
稳定性和存储Frozen in liquid nitrogen.
规格1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景

Insulin-like growth factor 1 receptor (CD221/IGF1R) is a ~154 kDa protein. Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins.

图片

  • Flow cytometry

    Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IGF1R monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
产品说明书
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
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公式
C₁ × V₁ = C₂ × V₂
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母液
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V₁ (起始体积)
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工作液
C₂ (终浓度)
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V₂ (终体积)

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HEK293T Human CD221/IGF1R Stable Cell Line

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