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HEK293T Cell Line (HG001818)

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概述
货号HG001818
品牌abinScience
描述
HEK293T Cell Line (HG001818) is a parental HEK293T cell line. Widely used as a host cell for transient transfection, stable cell line generation, and recombinant protein production.
Highlights
  • ●Parental Cell Line — Unmodified HEK293T cells suitable as host cells or negative controls.
  • ●High Transfection Efficiency — Contains the SV40 large T antigen for enhanced episomal replication of plasmids carrying the SV40 origin.
  • ●Versatile Applications — Ideal for transient transfection, lentiviral packaging, stable cell line development, and recombinant protein expression.
应用FCM
种属Human
检测方法Colorimetric
样本类型Plasma, Serum
实验类型Quantitative
检测范围1.56 - 100 ng/mL
灵敏度0.25 ng/mL
回收率80-120%
性能Growth Properties: Adherent; Morphology: Epithelial; Culture Medium: DMEM + 10% FBS + 1% Penicillin-Streptomycin; Culture Conditions: 37°C, 5% CO₂
运输Dry ice
稳定性和存储Frozen in liquid nitrogen.
规格1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
别名293T, HEK-293T, HEK 293T
背景

HEK293T is a highly transfectable derivative of the human embryonic kidney 293 (HEK293) cell line that stably expresses the SV40 large T antigen. The presence of the T antigen allows episomal replication of transfected plasmids containing the SV40 origin of replication, leading to amplification of the plasmid and extended temporal expression of the desired gene products. HEK293T cells are widely used for transient protein expression, lentiviral and retroviral packaging, and as host cells for generating stable cell lines overexpressing specific target genes. They grow as an adherent monolayer and are readily maintained in standard culture conditions.

NoteFor research use only
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公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
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浓度
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体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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