Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:2000-1:20000
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景
Granzyme B (GZMB) is a ~27 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis.
1. Poe, M. et al. (1991) The Journal of biological chemistry 266, 98-103. PMID: 1985927
2. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682
3. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427
4. Froelich, CJ. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 7161-71. PMID: 8258716
5. Liu, Y. et al. (2020) Science immunology 5. PMID: 31953257
6. Zhang, Z. et al. (2020) Nature 579, 415-420. PMID: 32188940
7. Yang, X. et al. (1998) The Journal of biological chemistry 273, 34278-83. PMID: 9852092
SEC-HPLC detection for Anti-Human GZMB Antibody (ABS0372).
SDS-PAGE
SDS-PAGE for Anti-Human GZMB Antibody (SAA0515).
Western blot
Western blot analysis was performed using anti-GZMB monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human GZMB (Catalog No: HY574012) Lane 2: negative control
