Anti-Rat VEGFA Recombinant Antibody (B20-4) (RB941013)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

RB941013

abinScience

种属反应性

Human, Mouse, Rat, Rabbit

Blocking, ELISA

Mouse

Monoclonal

IgG2a, kappa

B20-4

经测试应用

WB, FCM

L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor

内毒素水平

< 10 EU/mg

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P16612

Anti-Rat VEGFA Antibody (B20-4) (abinScience Cat# RB941013, RRID:AB_3727198)

Liquid

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Vascular endothelial growth factor A (VEGFA) is a ~25 kDa protein. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. VEGFA is the therapeutic target of bevacizumab (Avastin).

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Bioactivity

Detects Rat VEGFA in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Rat VEGFA Antibody (B20-4).
Flow CytoMetry

Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011)
Lane 2: negative control

产品说明书

Anti-Rat VEGFA Recombinant Antibody (B20-4).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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实验相关试剂

Anti-Rat VEGFA Recombinant Antibody (B20-4) (RB941013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Rat VEGFA Recombinant Antibody (B20-4) (RB941013)

概述

图片

参考文献

产品说明书

相关产品推荐

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