Anti-Human POLR1A Recombinant Antibody (SAA1537) (HF629013)

价格:

规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HF629013

abinScience

种属反应性

Human

ELISA

Mouse

Monoclonal

IgG2a, kappa

SAA1537

RPA194, POLR1A, RNA polymerase I 194 kDa subunit, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, A190, DNA-directed RNA polymerase I subunit A

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

O95602

Anti-Human POLR1A Antibody (SAA1537) (abinScience Cat# HF629013, RRID:AB_3727298)

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

DNA-directed RNA polymerase I subunit RPA1 (POLR1A) is a ~194 kDa protein. Catalytic core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Transcribes 47S pre-rRNAs from multicopy rRNA gene clusters, giving rise to 5.8S, 18S and 28S ribosomal RNAs. Pol I-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol I pre-initiation complex (PIC) is recruited by the selectivity factor 1 (SL1/TIF-IB) complex bound to the core promoter that precedes an rDNA repeat unit. The PIC assembly bends the promoter favoring the formation of the transcription bubble and promoter escape.

1. Miller, G. et al. (2001) The EMBO journal 20, 1373-82. PMID: 11250903

2. Panov, KI. et al. (2001) Molecular and cellular biology 21, 2641-9. PMID: 11283244

3. Panov, KI. et al. (2006) The EMBO journal 25, 3310-22. PMID: 16858408

4. Zhao, D. et al. (2021) Cell discovery 7, 97. PMID: 34671025

5. Misiaszek, AD. et al. (2021) Nature structural & molecular biology 28, 997-1008. PMID: 34887565

6. Daiß, JL. et al. (2022) Life science alliance 5. PMID: 36271492

For research use only.

SDS-PAGE

SDS-PAGE for Anti-Human POLR1A Antibody (SAA1537)

产品说明书

Anti-Human POLR1A Recombinant Antibody (SAA1537).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLR1A Recombinant Antibody (SAA1537) (HF629013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLR1A Recombinant Antibody (SAA1537) (HF629013)

概述

图片

参考文献

产品说明书

相关产品推荐

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