Anti-RAD51 Polyclonal Antibody (HC389014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

HC389014

品牌

abinScience

描述

Anti-RAD51 Polyclonal Antibody (HC389014) is a rabbit polyclonal antibody detecting RAD51 in ELISA, IHC, WB. Suitable for Bovine, Dog, Cricetulus griseus, Chicken, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human RAD51 (Val174-Asp339).

靶标

HsRAD51, RAD51A, RECA, hRAD51, RAD51, RAD51 homolog A, DNA repair protein RAD51 homolog 1

纯化方式

Purified by antigen affinity column.

Accession号

Q06609

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA repair protein RAD51 homolog 1 is a ~36 kDa protein. Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Recruited to resolve stalled replication forks during replication stress. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR.

1. Sigurdsson, S. et al. (2002) The Journal of biological chemistry 277, 42790-4. PMID: 12205100

2. Park, JY. et al. (2008) Nucleic acids research 36, 3226-34. PMID: 18417535

3. Islam, MN. et al. (2010) Molecular and cellular biology 30, 2460-72. PMID: 20231364

4. Schwendener, S. et al. (2010) The Journal of biological chemistry 285, 15739-45. PMID: 20348101

5. Yata, K. et al. (2012) Molecular cell 45, 371-83. PMID: 22325354

6. Wan, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10646-51. PMID: 23509288

7. Yuan, J. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10640-5. PMID: 23754376

8. Ameziane, N. et al. (2015) Nature communications 6, 8829. PMID: 26681308

9. Inano, S. et al. (2017) Molecular cell 66, 622-634.e8. PMID: 28575658

10. Brannvoll, A. et al. (2020) Cell reports 32, 107849. PMID: 32640219

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-RAD51 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

参考文献

产品说明书

Anti-RAD51 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RAD51 Polyclonal Antibody (HC389014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RAD51 Polyclonal Antibody (HC389014)

概述

图片

参考文献

产品说明书

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