Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HC396014

品牌

abinScience

描述

Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014) is a rabbit polyclonal antibody detecting GOLGA2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human GM130/GOLGA2 (Ala361-Ala678).

靶标

130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, Golgin subfamily A member 2, GOLGA2

纯化方式

Purified by antigen affinity column.

Accession号

Q08379

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Golgin subfamily A member 2 (GM130/GOLGA2) is a ~113 kDa protein. Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization.

1. Puthenveedu, MA. et al. (2006) Nature cell biology 8, 238-48. PMID: 16489344

2. Wei, JH. et al. (2015) Cell 162, 287-299. PMID: 26165940

3. Rivero, S. et al. (2009) The EMBO journal 28, 1016-28. PMID: 19242490

4. Kodani, A. et al. (2008) Molecular biology of the cell 19, 745-53. PMID: 18045989

5. Kodani, A. et al. (2009) Molecular biology of the cell 20, 1192-200. PMID: 19109421

6. Marra, P. et al. (2007) Molecular biology of the cell 18, 1595-608. PMID: 17314401

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-GOLGA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K562 cell lysate

参考文献

产品说明书

Anti-GM130/GOLGA2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014)

概述

图片

参考文献

产品说明书

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