Anti-PDK1 Polyclonal Antibody (HS822014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HS822014

品牌

abinScience

描述

Anti-PDK1 Polyclonal Antibody (HS822014) is a rabbit polyclonal antibody detecting PDK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human PDK1 (Tyr233-Met430).

靶标

PDHK1, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

纯化方式

Purified by antigen affinity column.

Accession号

Q15118

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1) is a ~49 kDa protein. Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia.

1. Gudi, R. et al. (1995) The Journal of biological chemistry 270, 28989-94. PMID: 7499431

2. McFate, T. et al. (2008) The Journal of biological chemistry 283, 22700-8. PMID: 18541534

3. Hitosugi, T. et al. (2011) Molecular cell 44, 864-77. PMID: 22195962

4. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675

5. Kato, M. et al. (2007) Structure (London, England : 1993) 15, 992-1004. PMID: 17683942

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-PDK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: 293T cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Lncap cell lystae
Lane 5: Hela cell lysate

参考文献

产品说明书

Anti-PDK1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PDK1 Polyclonal Antibody (HS822014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PDK1 Polyclonal Antibody (HS822014)

概述

图片

参考文献

产品说明书

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