| 货号 | HS839014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 品牌 | abinScience | ||||||||
| 种属反应性 | Human, Mouse, Rat, Anopheles gambiae, Asterias rubens, Bos taurus, Caenorhabditis elegans, Cairina moschata, Chironomus thummi thummi, Danio rerio, Drosophila erecta, Drosophila hydei, Drosophila mauritiana, Drosophila melanogaster, Drosophila orena, Drosophila sechellia, Drosophila simulans, Drosophila teissieri, Drosophila yakuba, Chicken, Holothuria tubulosa, Macaca fascicularis, Marthasterias glacialis, Oncorhynchus mykiss, Patella granatina, Patiria pectinifera, Penaeus vannamei, Platynereis dumerilii, Pongo abelii, Psammechinus miliaris, Salmo trutta, Sipunculus nudus, Strongylocentrotus purpuratus, Tigriopus californicus, Urechis caupo, Xenopus laevis, Zhangixalus schlegelii | ||||||||
| 应用 | ELISA, IHC, WB | ||||||||
| 宿主 | Rabbit | ||||||||
| 同种型 | IgG | ||||||||
| 克隆类型 | Polyclonal | ||||||||
| 免疫原 | E. coli - derived recombinant Human HIST2H2BE/H2BC21 (Pro2-Lys126). | ||||||||
| 靶标 | H2B/q, Histone H2B-GL105, H2B-clustered histone 21, Histone H2B.q, H2BC21, Histone H2B type 2-E, HIST2H2BE, H2BFQ | ||||||||
| 纯化方式 | Purified by antigen affinity column. | ||||||||
| Accession号 | Q16778 | ||||||||
| 状态 | Liquid | ||||||||
| 保存溶液 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| 产品使用信息 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Note | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting HIST2H2BE (HS839014) at a concentration of 13.2 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of HIST2H2BE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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