Anti-PCK2 Polyclonal Antibody (HS850014)

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概述
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参考文献
产品说明书

概述

货号

HS850014

品牌

abinScience

描述

Anti-PCK2 Polyclonal Antibody (HS850014) is a rabbit polyclonal antibody detecting PCK2 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human PCK2 (Ala292-Met640).

靶标

PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PCK2

纯化方式

Purified by antigen affinity column.

Accession号

Q16822

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) is a ~70 kDa protein. Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis. Also acts as a serine/threonine-protein kinase: phosphorylates and activates ACSL4, thereby promoting ferroptosis.

1. Escós, M. et al. (2016) Biochemistry and biophysics reports 7, 124-129. PMID: 28955899

2. Li, Z. et al. (2024) Nature chemical biology 20, 1341-1352. PMID: 38720107

Note

For research use only.

图片

参考文献

产品说明书

Anti-PCK2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PCK2 Polyclonal Antibody (HS850014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PCK2 Polyclonal Antibody (HS850014)

概述

图片

参考文献

产品说明书

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