Anti-SUMF1 Polyclonal Antibody (HV152014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HV152014

品牌

abinScience

描述

Anti-SUMF1 Polyclonal Antibody (HV152014) is a rabbit polyclonal antibody detecting SUMF1 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human SUMF1 (Glu113-Ser356).

靶标

Formylglycine-generating enzyme, FGE, SUMF1, Sulfatase-modifying factor 1, C-alpha-formylglycine-generating enzyme 1

纯化方式

Purified by antigen affinity column.

Accession号

Q8NBK3

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Formylglycine-generating enzyme (SUMF1) is a ~40 kDa protein. Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent. 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile. Known substrates include GALNS, ARSA, STS and ARSE.

1. Cosma, MP. et al. (2003) Cell 113, 445-56. PMID: 12757706

2. Preusser-Kunze, A. et al. (2005) The Journal of biological chemistry 280, 14900-10. PMID: 15657036

3. Dierks, T. et al. (2005) Cell 121, 541-552. PMID: 15907468

4. Roeser, D. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 81-6. PMID: 16368756

5. Schlotawa, L. et al. (2011) European journal of human genetics : EJHG 19, 253-61. PMID: 21224894

6. Holder, PG. et al. (2015) The Journal of biological chemistry 290, 15730-15745. PMID: 25931126

Note

For research use only.

图片

参考文献

产品说明书

Anti-SUMF1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SUMF1 Polyclonal Antibody (HV152014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SUMF1 Polyclonal Antibody (HV152014)

概述

图片

参考文献

产品说明书

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