Anti-WWTR1 Polyclonal Antibody (HV017014)

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概述
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参考文献
产品说明书

概述

货号

HV017014

品牌

abinScience

描述

Anti-WWTR1 Polyclonal Antibody (HV017014) is a rabbit polyclonal antibody detecting WWTR1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human WWTR1 (Met1-Leu400).

靶标

Transcriptional coactivator with PDZ-binding motif, TAZ, WW domain-containing transcription regulator protein 1, WWTR1

纯化方式

Purified by antigen affinity column.

Accession号

Q9GZV5

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

WW domain-containing transcription regulator protein 1 (WWTR1) is a ~44 kDa protein. Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex.

1. Kanai, F. et al. (2000) The EMBO journal 19, 6778-91. PMID: 11118213

2. Lei, QY. et al. (2008) Molecular and cellular biology 28, 2426-36. PMID: 18227151

3. Lucci, V. et al. (2013) Gene 529, 300-6. PMID: 23911299

4. Di Palma, T. et al. (2009) Experimental cell research 315, 162-75. PMID: 19010321

5. Varelas, X. et al. (2008) Nature cell biology 10, 837-48. PMID: 18568018

Note

For research use only.

图片

参考文献

产品说明书

Anti-WWTR1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-WWTR1 Polyclonal Antibody (HV017014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-WWTR1 Polyclonal Antibody (HV017014)

概述

图片

参考文献

产品说明书

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