Anti-FIS1 Polyclonal Antibody (HV072014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

HV072014

品牌

abinScience

描述

Anti-FIS1 Polyclonal Antibody (HV072014) is a rabbit polyclonal antibody detecting FIS1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Human, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human FIS1 (Met1-Ala117).

靶标

Mitochondrial fission 1 protein, FIS1 homolog, Tetratricopeptide repeat protein 11, FIS1, TTC11, hFis1, TPR repeat protein 11

纯化方式

Purified by antigen affinity column.

Accession号

Q9Y3D6

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Mitochondrial fission 1 protein (FIS1) is a ~16 kDa protein. Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event. Also mediates peroxisomal fission. May act when the products of fission are directed toward mitochondrial homeostasis, mitophagy, or apoptosis.

1. James, DI. et al. (2003) The Journal of biological chemistry 278, 36373-9. PMID: 12783892

2. Yoon, Y. et al. (2003) Molecular and cellular biology 23, 5409-20. PMID: 12861026

3. Stojanovski, D. et al. (2004) Journal of cell science 117, 1201-10. PMID: 14996942

4. Losón, OC. et al. (2013) Molecular biology of the cell 24, 659-67. PMID: 23283981

5. Yu, T. et al. (2005) Journal of cell science 118, 4141-51. PMID: 16118244

6. Koirala, S. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, E1342-51. PMID: 23530241

7. Shen, Q. et al. (2014) Molecular biology of the cell 25, 145-59. PMID: 24196833

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-FIS1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: PC-12 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse heart lysate
Lane 8: Mouse spleen lysate
Lane 9: Rat brain lysate
Lane 10: Rat heart lysate
Lane11: Rat spleen lysate

参考文献

产品说明书

Anti-FIS1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-FIS1 Polyclonal Antibody (HV072014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-FIS1 Polyclonal Antibody (HV072014)

概述

图片

参考文献

产品说明书

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