Anti-TAP1 Polyclonal Antibody (HX909014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HX909014

品牌

abinScience

描述

Anti-TAP1 Polyclonal Antibody (HX909014) is a rabbit polyclonal antibody detecting TAP1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human TAP1 (Tyr525-Glu808).

靶标

TAP1, APT1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, Peptide transporter TAP1, Peptide transporter PSF1, RING4, Antigen peptide transporter 1, PSF-1, ABCB2, Y3, Peptide supply factor 1, PSF1, ATP-binding cassette sub-family B member 2

纯化方式

Purified by antigen affinity column.

Accession号

Q03518

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Antigen peptide transporter 1 (TAP1) is a ~80 kDa protein. ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules.

1. Grossmann, N. et al. (2014) Nature communications 5, 5419. PMID: 25377891

2. Fischbach, H. et al. (2015) Nature communications 6, 6199. PMID: 25656091

3. Gorbulev, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 3732-7. PMID: 11274390

4. van Endert, PM. et al. (1995) The Journal of experimental medicine 182, 1883-95. PMID: 7500034

5. Uebel, S. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 8976-81. PMID: 9256420

6. Kelly, A. et al. (1992) Nature 355, 641-4. PMID: 1538751

Note

For research use only.

图片

参考文献

产品说明书

Anti-TAP1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-TAP1 Polyclonal Antibody (HX909014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-TAP1 Polyclonal Antibody (HX909014)

概述

图片

参考文献

产品说明书

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