ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
33.22 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
TAP1, APT1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, Peptide transporter TAP1, Peptide transporter PSF1, RING4, Antigen peptide transporter 1, PSF-1, ABCB2, Y3, Peptide supply factor 1, PSF1, ATP-binding cassette sub-family B member 2
背景
Antigen peptide transporter 1 (TAP1) is a ~80 kDa protein. ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules.
1. Grossmann, N. et al. (2014) Nature communications 5, 5419. PMID: 25377891
2. Fischbach, H. et al. (2015) Nature communications 6, 6199. PMID: 25656091
3. Gorbulev, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 3732-7. PMID: 11274390
4. van Endert, PM. et al. (1995) The Journal of experimental medicine 182, 1883-95. PMID: 7500034
5. Uebel, S. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 8976-81. PMID: 9256420
6. Kelly, A. et al. (1992) Nature 355, 641-4. PMID: 1538751
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human TAP1 Protein, N-His
