Anti-RPS6KA1 Polyclonal Antibody (HS750014) is a rabbit polyclonal antibody detecting RPS6KA1 in ELISA, IHC, WB. Suitable for Danio rerio, Chicken, Human, Mouse, and Rat.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human RPS6KA1 (Phe62-Tyr321).
靶标
p90-RSK 1, Ribosomal S6 kinase 1, RSK1, p90RSK1, RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, RSK-1, MAPKAPK1A, MAP kinase-activated protein kinase 1a, MAPKAPK-1a, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase alpha-1, p90S6K
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Ribosomal protein S6 kinase alpha-1 (RPS6KA1) is a ~82 kDa protein. Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex.
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