Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Ribosomal protein S6 kinase alpha-1 (RPS6KA1) is a ~82 kDa protein. Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex.
1. Shimamura, A. et al. (2000) Current biology : CB 10, 127-35. PMID: 10679322
2. Wu, J. et al. (2002) The Journal of biological chemistry 277, 42669-79. PMID: 12213813
3. Hu, Y. et al. (2004) The Journal of biological chemistry 279, 29325-35. PMID: 15117958
4. Wingate, AD. et al. (2006) The Biochemical journal 393, 715-24. PMID: 16223362
5. Roux, PP. et al. (2007) The Journal of biological chemistry 282, 14056-64. PMID: 17360704
6. Carrière, A. et al. (2008) Current biology : CB 18, 1269-77. PMID: 18722121
7. Zhou, Y. et al. (2015) Nature communications 6, 7679. PMID: 26158630
